Thanks for using MG-RAST.

You need to send the COG proteins to the workbench.
http://blog.metagenomics.anl.gov/howto/using-the-workbench/

On the workbench page, below the table, is a button labeled
download metagenome dna FASTA annotated by COG, which
generates a fasta file like this:

>4440275.3|228555_3152_1807|COG|COG0489 ATPases involved in chromosome partitioning
catctaataaagcaacatcttgaggtgttgaatacaataacagcacccgctaaatttact
tgttgtgccaatgatatctgtatatctccagtcccaggtggaagatcaataataattata
tctaaattgccccatgctacagaatttagcatttgagttaaggcagactgtaccataggt
cctcgccaaatcattgctgtgtcgtcaggaactaatagccccatagacatcaaacttatt
cctaaaa
>4440275.3|318862_2493_3356|COG|COG1192 ATPases involved in chromosome partitioning
ggcactcacgttgagccccgatttatacaaaaaacagatattgaaaatatcgatcttgtc

containing the sequences that had similarity matches.

I hope this helps.

William Trimble
for the MG-RAST team

---------- Forwarded message ----------
From: <Dongmei.Li@csiro.au>
Date: Mon, Jun 27, 2011 at 3:23 AM
Subject: [mg-rast] request
To: mg-rast@mcs.anl.gov


Dear Sir/Madam,

 

Sorry to bother you .

I am using COG analysis in MG-RAST,  for example, I have got the information below:

metagenome

level 1

level 2

function

id

abundance

avg eValue

avg % ident

avg align len

# proteins

4448084.3

CELLULAR PROCESSES AND SIGNALING

Cell cycle control, cell division, chromosome partitioning

Integral membrane protein possibly involved in chromosome condensation

COG0239

112

-12.5

74.38

48.79

14

 

My question is whether I can get the information about which reads of the 454 sequences are assigned to COG0239?

 

Thank you in advance,

 

Regards,

Dongmei

 

Dr. Dongmei Li
Research Scientist| MEOR

CSIRO Food & Nutritional Sciences

Phone: +61 2 9490 5078 | Fax: +61 2 9490 5010
dongmei.li@csiro.au | www.csiro.au | www.csiro.au/CFNS
Address: 11 Julius Ave. North Ryde,NSW 2113

PLEASE NOTE
The information contained in this email may be confidential or privileged. Any unauthorised use or disclosure is prohibited. If you have received this email in error, please delete it immediately and notify the sender by return email. Thank you. To the extent permitted by law, CSIRO does not represent, warrant and/or guarantee that the integrity of this communication has been maintained or that the communication is free of errors, virus, interception or interference.

Please consider the environment before printing this email.