Hi, The demultiplexing should not take long, a couple of minutes for small files, more for large files. I will take a look and see what the problem is. There is some documentation at: http://blog.metagenomics.anl.gov/howto/ http://blog.metagenomics.anl.gov/mg-rast-v3-0-faq/ Regards, Mark D'Souza -- for the MG-RAST team [email protected] http://metagenomics.anl.gov/ If you need to respond to this email please address it to the mailing-list [email protected] On Jun 1, 2011, at 9:03 AM, [email protected] wrote:

I currently started using the MG-RAST program to process multiplexed 454-amplicon sequences. We have uploaded a 454 .sff file and we then started using the MG-RAST QC pipeline. I also tried to use the "de-multiplexing" option to separate the differentially MID-labeled amplicons. Is there any special rule for filling in the MID sequences that are used in the experiment in the corresponding field? I have just filled in the used MID sequences and separated these individual MID's by a "hard return", is that correct? After starting the uploading and processing pipeline, the following messages appear under the "current uploads" tab: Filename, Status: (Demultiplexing started), Next step: (demultiplexing completed), progress: (demultiplexing.....). The program does not seem to get any further. Am I doing something wrong, or should I be more patient? Is there a more extensive MG-RAST manual for beginners available?

Thank you for your help in advance.

Kind regards, Leo Heijnen