Hi, I am fiddling around with MG-RAST and have come across a bit of a problem when using M5NR as my annotation source. If I try generating a bar chart of any metagenomic data under the organism classification tab using this M5NR database, whether it's my own or a public dataset/s, I keep getting no bars in my graph - they are all zero! When I try selecting my group of annotation sources as all of the databases in the M5NR (i.e SwissProt, Genbank etc etc) then I get some lovely looking bars on my chart. I'm assuming this is a bug with the M5NR database since the organism classification using exactly the same databases all together result in a proper chart being generated, so I thought I'd pass this on. If it isn't a bug and I'm just not doing it properly then I apologise and look forward to getting the proper method from you guys! Other than this problem, so far I've found MG-RAST to be very valuable in my analysis, although it is only at the preliminary stages! So, thank you for producing such a user-friendly and robust program Cheers, Sophie Davidson Sophie Davidson | BioScience Researcher | BioScience Division Department of Primary Industries | Victorian AgriBiosciences Centre | 1 Park Dr | Bundoora VIC 3083 T: 03 9032 7111 | F: 03 9479 3618 | E: [email protected] Notice: This email and any attachments may contain information that is personal, confidential, legally privileged and/or copyright.No part of it should be reproduced, adapted or communicated without the prior written consent of the copyright owner. It is the responsibility of the recipient to check for and remove viruses. If you have received this email in error, please notify the sender by return email, delete it from your system and destroy any copies. You are not authorised to use, communicate or rely on the information contained in this email. Please consider the environment before printing this email.